One of the most important fields of study in medical science is to develop new and improved ways in which to diagnose diseases. The earlier a disease can be diagnosed, the easier it is to treat. Early diagnosis also improves the prognosis. This is why so many scientists concentrate upon the development of new and more efficient tests. One such test that has proven to be invaluable is the enzyme linked immunosorbent assay. With ELISA kits doctors can now diagnose various conditions easily and efficiently.
Also known as enzyme immunoassay, or EIA for short, this laboratory technique is used to measure the concentration of antibodies or antigens in a solution, normally a blood sample. The ability to determine these concentrations is extremely important in the diagnostic process. Among the main advantages of this technique, when compared with others, is that it is very easy to carry out and a large number of tests can be processed simultaneously.
The ability to identify the presence of either antibodies or antigens in the body is very important. This is because antigens are known to be harmful substances. As soon as the body recognizes the presence of antigens, it starts to produce antibodies to neutralize the harmful antigens. When either of these agents is present in a sample, the existence of a disease can be deduced logically.
There is no need to stress about this type of test. Patients are required to provide a small blood sample. This sample is then added to a petri dish that contains antigens typical to the condition for which the patient is tested. If the blood sample combines with the antigen, antibodies are present and this confirms the diagnosis. The amount of antibodies is determined by carefully adding enzymes to the sample.
The EIA test provides a wealth of information, even when administered in its simplest form. However, more complex techniques can provide even better results. Different formats, or kits, are used for the different forms of the test. For example, in the direct EIA test the antigen is coated with a multiwall plate. The antibody can only detect it if the latter has been conjugated with an enzyme.
Another important variant of EIA is the indirect test. In this case two stages are engineered by covering the antigens in polystyrene plates. This is done to achieve increased sensitivity. Antibodies are introduced in primary and secondary stages. The test can produce multiple results which means that is saves cost and time. In many cases this test, which is more elaborate, eliminate the need for more tests.
The sandwich EIA test needs pairs of antibodies that are matched in pairs. The first antibody is coated with polystyrene. A second antibody then serves as the detection agent. The purpose of this test is to determine the concentration of the analyte. This test is extremely useful in the diagnosis of latent diseases and it is deemed to be highly accurate.
Nobody will deny the fact that diseases are easier and cheaper to treat if they can be diagnosed at an early stage. Tests such as the EIA help doctors to do just this. Conditions such as HIV, pernicious anemia, syphilis, and many others have been diagnosed early enough to save the lives of the patient, all thanks to this amazing procedure.
Also known as enzyme immunoassay, or EIA for short, this laboratory technique is used to measure the concentration of antibodies or antigens in a solution, normally a blood sample. The ability to determine these concentrations is extremely important in the diagnostic process. Among the main advantages of this technique, when compared with others, is that it is very easy to carry out and a large number of tests can be processed simultaneously.
The ability to identify the presence of either antibodies or antigens in the body is very important. This is because antigens are known to be harmful substances. As soon as the body recognizes the presence of antigens, it starts to produce antibodies to neutralize the harmful antigens. When either of these agents is present in a sample, the existence of a disease can be deduced logically.
There is no need to stress about this type of test. Patients are required to provide a small blood sample. This sample is then added to a petri dish that contains antigens typical to the condition for which the patient is tested. If the blood sample combines with the antigen, antibodies are present and this confirms the diagnosis. The amount of antibodies is determined by carefully adding enzymes to the sample.
The EIA test provides a wealth of information, even when administered in its simplest form. However, more complex techniques can provide even better results. Different formats, or kits, are used for the different forms of the test. For example, in the direct EIA test the antigen is coated with a multiwall plate. The antibody can only detect it if the latter has been conjugated with an enzyme.
Another important variant of EIA is the indirect test. In this case two stages are engineered by covering the antigens in polystyrene plates. This is done to achieve increased sensitivity. Antibodies are introduced in primary and secondary stages. The test can produce multiple results which means that is saves cost and time. In many cases this test, which is more elaborate, eliminate the need for more tests.
The sandwich EIA test needs pairs of antibodies that are matched in pairs. The first antibody is coated with polystyrene. A second antibody then serves as the detection agent. The purpose of this test is to determine the concentration of the analyte. This test is extremely useful in the diagnosis of latent diseases and it is deemed to be highly accurate.
Nobody will deny the fact that diseases are easier and cheaper to treat if they can be diagnosed at an early stage. Tests such as the EIA help doctors to do just this. Conditions such as HIV, pernicious anemia, syphilis, and many others have been diagnosed early enough to save the lives of the patient, all thanks to this amazing procedure.
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